大神棋牌

上海远慕生物科技有限公司

植物体内脱落酸、赤霉素的分离和测定
点击次数:29 发布时间:2020-07-23

ZAIYANJIUZHIWUSHENGMINGHUODONGGUOCHENGZHONG,CHANGCHANGXUYAOZHUNQUELEJIEMOUYIJISUDEHANLIANGYIJIGEJISUJIANDEBILI。YINCI,ZHIWUNEIYUANJISUDETIQUFENLIHECEDINGSHIZHIWUSHENGLIXUESHIYANJISHUZHONGJIQIZHONGYAODENEIRONG。BENSHIYANYIABAHEGAWEILI,JIESHAOJISUDETIQU、FENLIJICEDINGDEJIBENYUANLIHEFANGFA。

YI、YUANLI

大神棋牌LIYONGTUOLUOSUAN(abscisic acid,ABA)HECHIMEISU(gibberellic acid,GA)NENGRONGJIEYUYOUJIRONGJI(RUBINGTONG、JIACHUN)DETEXINGJINXINGTIQU,JIANGCUTIWUJINGGUOYIXILIEDEFENLIJISHU(RUCUIQU、BOCENGCENGXIHUOZHICENGXIDENG),SHIABA、GAYUQITACHENGFENFENLI。ZAIDUICHUNHUADEABAHEGAJINXINGSHENGWUXUEJIANDINGHUOWULIHUAXUEJIANDING。

(YI)SHENGWUJIANDING1.ABANENGYIZHIZHIWUQIGUANDESHENGZHANG,ZAIYIDINGDENONGDUTIAOJIANXIA,QIYIZHICHENGDUYUNONGDUCHENGXIANXINGGUANXI。LIYONGZHEIYIXIANXINGGUANXIJIUKEQUEDINGZUZHIZHONGABADEHANLIANG。2.GANENGCIJIYOUNENZHIWUDEJIEJIANSHENZHANG,TEBIESHIAISHENGZHIWUDEJING。ZAIYIDINGNONGDUFANWEINEI,JINGDESHENZHANGDUYUGANONGDUCHENGXIANXINGGUANXI。YINCI,GENJUJINGDESHENZHANGDUJIUKEQUEDINGGADEHANLIANG。

(ER)WULIHUAXUEJIANDING:KECAIYONGQIXIANGSEPUFA。

 

 

大神棋牌ER、CAILIAO、YIQISHEBEIJISHIJI

(YI)CAILIAO:ZHIWUYEPIAN、SHUIDAOZHONGZIJIYOUMIAODENG;

(ER)YIQISHEBEI:1.QIXIANGSEPUYI;2.FENYELOUDOU;3.ZUZHIYUNJIANGQI;4.XUANZHUANZHENGFAGANZAOQI;5.CENGXIGANG;6.HUATEMAN-3HAOCENGXIZHI;7.WEILIANGZHUSHEQI。

(SAN)SHIJI:1. 100%JIACHUN;2. 80%JIACHUN;3. SHIYOUMI;4. YISUANYIZHI;5. 1mol/LHCl;6. GUISUANGF232;7. LV-FANG;8. GA;9. ABA;10. YICHUN;11. ZHENGDINGCHUN;12. YIBINGCHUN;13. 3mol/LANSHUI。

SAN、SHIYANBUZHOUYUJIEGUOJISUAN

(YI)TUOLUOSUANHECHIMEISUDETIQUHEFENLI

1.YANGPINTIQU(1)QUXINXIANCAILIAOHUOZHUCUNCAILIAO(YONG100%JIACHUNGUDING,FANGZHI-10℃BINGXIANGZHONGZHIDAICESHI)10g,JIANSUI,JIARU60mlYULENG(<0℃=DE80%JIACHUNRONGYE,YUNJIANG5min,YUNJIANGYEZAI4℃XIAZHENDANG(HUOJIAOBAN)24h,GUOLV。CANZHAZAIYONG20mlJIACHUNYEZHENDANG1h。FANFUERCI,LVYEHUNHE。(2)JIANGLVYEZAIXUANZHUANZHENGFAQISHANGGANZAOJIANYAZHENGFA(36~38℃)ZHIYUANTIJIYIBAN,ZAIJIARU10mlSHIYOUMITIQUBUFENSESU,JIANGXIACENGJIACHUNYEQUCHU,QIDIAOSHIYOUMICENG,JIXUJIANZHINONGSUOZHISHUIRONGYE。ZAICISHUIRONGYEZHONGHANYOUIAA、ABA、GAHECTKDENG。

大神棋牌2.YANGPINCUIQUJIANGSHUIRONGYEYONG1mol/LHClDIAOpHZHI2.8~2.5ZUOYOU。BINGYIYUSHUIRONGYEDENGTIJIDEYISUANYIZHICUIQU。JIANGHUNHERONGYEZHUANGRUFENYELOUDOU,FENLISHUIXIANGHEYISUANYIZHIXIANG。QUSHUIXIANGZAIYONGYISUANYIZHICUIQU2CI。HEBINGYISUANYIZHIRONGYE。CISHI,CTK(XIBAOFENLIESU)CUNZAIYUSHUIXIANGZHONG,ERABA、GA、IAACUNZAIYUYISUANYIZHIZHONG。

大神棋牌3.YANGPINDECHUNHUA(1)JIANGYISUANYIZHIRONGYEYONGXUANZHUANZHENGFAQINONGSUOZHIGAN。YONG2ml100%JIACHUNRONGJIECANLIUWU。(2)DIANYANG:QU100μlJIACHUNRONGYEDIANZAITUYOUGUIJIAOGF254BOBANXIADUAN1cmCHU(HUODIANZAIHUATEMAN3HAOCENGXIZHISHANG),BINGZAITONGYISHUIPINGSHANGFENBIEDIANSHANGBIAOZHUNGAHEABARONGYE100μl。(3)ZHANCENG:YONGYIBINGCHUN-28%ANSHUI-SHUI(10∶1∶1,V/V/V)ZUOWEIZHANCENGJIZHANCENG。QIANYANZHIBOBANDINGDUAN0.5cmCHUJITINGZHIZHANCENG。(4)DINGWEI:CENGXIWANBIHOU,CHUIGANBOBANBINGZAIZIWAIDENGXIAGUANCHASEDAI,YUBIAOZHUNABAHEGARfZHIXIANGTONGHUOXIANGJINDESEDAI,JIUZUOWEICHUNHUAHOUDEABAHEGA。(5)XITUO:JIANGABAHEGADAIFENBIEKUOXIA(HUOJIANXIA),YONG95%YICHUNJINTI3CI。RONGYEJINGJIANYAZHENGGANHOU,JIKEJINYIBUZUOSHENGWUXUECEDINGHUOQIXIANGSEPUCEDINGZHIYONG。

(ER)ABAHEGADECEDING

大神棋牌1.ABADESHENGWUXUEJIANDING

大神棋牌(1)CAILIAOPEIYANG:JINGXUANXIAOMAIZHONGZI,25℃HEIANXIAJINZHONG2h,PAIYUPEIYANGMINSHILVZHISHANG,ZAI25℃XIAFAYA。DAIPEIGENCHUXIANHOU,YIRUPEIYANGGANGZHONGDESULIAOWANGSHANG,JIXUZAI25℃ANZHONGPEIYANG。YUE72hHOU,XUANQUPEIYAQIAO2.8~3.0cmDEYOUMIAO,YONGDAOPIANZIDINGDUANFENBIEQIECHENG3mm,5mm,5mmSANXIAODUAN,QUZHONGJIAN5mmQIEDUANZHIZHENGLIUSHUIZHONG2~3h,BEIYONG。

大神棋牌(2)ABAMUYE(200μg/ml)DEPEIZHI:CHENGQU20mgABA,RONGYUSHAOLIANGJIUJING,YIWULIZISHUIDINGRONGZHI100ml。

(3)BIAOZHUNRONGYEDEPEIZHI:XIQU5mlMUYE,JIAWULIZISHUIDINGRONGZHI100ml,JIWEI10μg/ml。XIQU5ml10μg/mlABARONGYE,YONG2%ZHETANG-0.01mol/LLINSUANHUANCHONGYE(pH5.0)DINGRONGZHI50ml,JIWEI1μg/mlABABIAOZHUNYE。YUCILEITUI,FENBIEPEICHENG0、0.001、0.01、0.1、1μg/mlBIAOZHUNYE。

(4)BIAOZHUNQUXIANDEHUIZHI:QU2mlGEZHONGNONGDUDEABABIAOZHUNYEFENBIEZHIRU10mlJUSAISHIGUAN,MEIGUANJIARUXIAOMAIYAQIAOQIEDUAN10GEN。GENONGDUJUNXU3~4GEZHONGFU。JIASAIHOU,ZHIANCHU25℃YAOCHUANGSHANGZHENDANG20h。JIANG10GEQIEDUANQUCHUCEDINGQIZONGZHANGDU(cm),RANHOUANXIALIEGONGSHIJISUANCHULIHOUJIANSHAODEBAIFENSHU(R):R(%)=(DKONG-DCHULI)/DYUAN×100。

SHIZHONG:DKONG:KONGBAIZONGZHANGDU;DCHULI:CHULIZONGZHANGDU;DYUAN:YUANSHIZONGZHANGDU(5.0cm)。YONGRYUABANONGDUDEXIANGGUANXING,HUIZHIBIAOZHUNQUXIAN。

大神棋牌(5)JIANGDAICEYANGPINRONGYU2ml2%ZHETANG-0.01mol/LLINSUANHUANCHONGYE(pH5.0),ZHIJUSAISHIZHONG,ANSHANGSHUBUZHOUCAOZUO,SUANCHURZHI,ZAICHABIAOZHUNQUXIAN,JIUDEDAOABANONGDUC,YIXIASHIJISUANCHUYANGPINZHONGABAHANLIANG:ABAHANLIANG(μg/gXIANZHONG)=C(μg/ml×2×10-1/W(g)2.ABADEQIXIANGSEPUCEDINGJIANGXITUODEABAJIARU0.1mlBSA(N.O-ER-SANJIAJIGUIWANJIYIXIANAN)SHITUOLUOSUANGUIWANHUA。0.5hHOU,XIQU5μlZUOQIXIANGCENGXI,CENGXITIAOJIAN:ZHUZHANG2m,φ5mm(BUXIUGANGZHU)、GUDINGXIANGWEI10%SE-30,DMCS、A、WWEIDANTI,JINYANGKOUWENDU250℃,ZHUWEN200℃,FIDJIANCE。ZAIQILIUSUWEI30μl/min,CAIYONGNEIBIAOFADINGXING,WAIBIAOFADINGLIANG。JISUAN:ABAHANLIANG(μg/gXIANZHONG)=A1×Ws×100×1/(As×5×W)

 

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