大神棋牌

上海远慕生物科技有限公司

免疫组化方法中关键环节及其原理解述
点击次数:38 发布时间:2020-07-29

YI、MEIMIANYIZUHUADEGUANJIANHUANJIE

1、BIAOBENGUDING:GUDINGDEMUDESHI①FANGZHIBIAOBENCONGBOPIANSHANGTUOLUO;②CHUQUFANGAIKANGYUAN-KANGTIJIEHEDELEIZHI,SHIKANGYUANKANGTIJIEHEWUYIYUHUODELIANGHAODERANSEJIEGUO;③GUDINGDEBIAOBENYIYUBAOCUN。

2、TUOSHUI、SHILABAOMAIHEZHIPIAN:TUOSHUIYONGTIDUYICHUN(YOUDIDAOGAO)CHONGFENTUOSHUI、DUIZUZHIYAOWANQUANJINLA、QIEPIANSHIDAOPIANYAOGANJINGHEFENGLI。FOUZE,RONGYILIEPIANHETUOPIANDENG。

大神棋牌3、TUOLAHESHUIHUA:ZHEISHIWEILEHOUMIANDEKANGTIDENGSHIJINENGGOUCHONGFENYUZUZHIZHONGKANGYUANDENGJIEHEFANYING。TUOLAKEYIXIAN60DU20min,RANHOULIJIERJIABEN1-3FENBIE10min,DANDANGTIANZHIHAODEQIEPIANKEYI60DU3-4h。SHUIHUAYONGTIDUYICHUN(YOUGAODAODI)。RUOTUOLAHESHUIHUABUQUANYICHUXIANJUZAOXINGFANYINGHEJINXIBUQUAN,ERCHANSHENGFEITEYIXINGBEIJINGZHESE。

4、KANGYUANXIUFU:YOUYUZUZHIZHONGBUFENKANGYUANZAIJIAQUANHUODUOJUJIAQUANGUDINGGUOCHENGZHONG,FASHENGLEDANBAIZHIJIANJIAOLIANJIQUANJIDEFENGBIZUOYONG,CONGERSHIQUKANGYUANXING;TONGGUOKANGYUANXIUFU,SHIDEXIBAONEIKANGYUANJUEDINGZUZHONGXINBAOLU,TIGAOKANGYUANJIANCELV。CHANGYONGDEXIUFUFANGFACONGQIANGDAORUOYIBANFENWEISANZHONG,GAOYAXIUFU、WEIBOXIUFU、YIMEIXIUFU。XIUFUYEYEFENWEIRUOGANZHONG(JUTIDEKEYICHAYUEXIANGGUANZILIAO,DALIANGDE:ZHONGXINGDE、GAOpHDEDENG)。WOMENSHIYANSHIYIBANYONGWEIBOXIUFUZHONGHUO6min*4CI,XIAOGUOBUCUO。ZHUYIWEIBOXIUFUHOUZIRANLENGQUE30minZUOYOU(ZHIYAONIJUEDEXIUFUYEDEWENDUDASHIWENJIKE)。

5、XIBAOTONGTOU:MUDESHISHIKANGTINENGGOUCHONGFENDIJINRUBAONEIJINXINGJIEHEFANYING。YIBANYONGTriton X-100、DANBAIMEIkDENGTONGTOUYE。RUTriton X-100KEYIRONGJIEXIBAOMO、XIBAOHEMO、XIBAOQIMOSHANGDEZHIZHIERSHIKANGTIJIDAFENZIJIEGOUDEWUZHIJINRUBAOJIANGHEBAOHENEI,GUZAIXIBAOMIANYIZUHUASHIYOUWEITUIJIANSHIYONG,ZHEIYANGKANGTIJIUNENGSHUNLIJINRUBAONEIYUXIANGYINGKANGYUANJIEHE。ZAIMIANYIZUZHIHUAXUE(>10umHOUQIEPIAN) HEMIANYIXIBAOHUAXUEZHONGYIBANYONGTriton X-100 ZUOWEIXIBAOTONGTOUJI,ZAIMOSHANGDAKONG。

6、MIEHUONEIYUANXINGGUOYANGHUAWUMEIHESHENGWUSU:ZAICHUANTONGDEABCFAHESPFAZHONG,MIANYIZUHUAFANYINGJIEGUORONGYISHOUDAONEIYUANXINGGUOYANGHUAWUMEIHESHENGWUSUDEGANRAO,BIXUYONGGUOYANGHUAQINGHELUANBAISUDENGJINXINGMIEHUO。MIEHUONEIYUANXINGPODYIBAN3%GUOYANGHUAQINGMIEHUOSHIJIANDUANDIAN,KEYI10minZUOYOU,ER0。3%GUOYANGHUAQINGZEKEYISHIDANGYANZHANGFENGBISHIJIAN,YIBAN10~30min;YONGJIACHUNPEIZHIGUOYANGHUAQINGBISHUANGZHENGSHUIHUOPBSKENENGHAOZAIBAOHUKANGYUANHEGUDINGZUZHIZUOYONG,GUOYANGHUAQINGFUYUSHIJIANGUOZHANGYIYINQITUOPIAN;XIANYONGXIANPEI,PEIHAOHOU4DUBIGUANGBAOCUN。BUGUO,XIANZAIYIYOU“DIERDAIJIYONGXINGMIANYIZUHUASHIJIHE”BIMIANNEIYUANXINGSHENGWUSUDEGANRAO,TUIJIANSHIYONG。

大神棋牌7、XUEQINGFENGBI:ZUZHIQIEPIANSHANGYOUSHENGYUDEWEIDIANKEYIYUYIKANGFEITEYIXINGJIEHE,ZAOCHENGHOUXUJIEGUODEJIAYANGXING;FENGBIXUEQINGYIBANSHIHEERKANGTONGYILAIYUANDE,XUEQINGZHONGDONGWUZISHENDEKANGTI,YUXIANNENGHEZUZHIZHONGYOUJIAOCHAFANYINGDEWEIDIANFASHENGJIEHE;YEKEYIYONGXIAONIUXUEQING、BSA、YANGXUEQINGDENG,DANBUNENGYUYIKANGLAIYUANYIZHI。YIBANSHIWENHUO37DU10-30min。

8、YIKANGHEERKANGNONGDUHEFUYUSHIJIAN:YIKANGFUYUTIAOJIANZAIMIANYIZUHUAFANYINGZHONGZUIZHONGYAO,BAOKUOFUYUSHIJIANHEKANGTINONGDU。YIKANGFUYUWENDUYOUJIZHONG:4DU、SHIWEN、37DU,QIZHONG4DUXIAOGUOZUI-JIA;FUYUSHIJIAN:ZHEIYUWENDU、KANGTINONGDUYOUGUAN,YIBAN37DU1-2h,ER4DUGUOYEHECONGBINGXIANGNACHUHOU37DUFUWEN45min。JUTITIAOJIANHAIYAOMOSUO。ERKANGFUYUTIAOJIAN:ERKANGYIBANSHIWENHUO37DU30min-1h,JUTISHIJIANXUYAOMOSUO,ERNONGDUYIBANYOUGONGZUOYE,RUOSHINONGSUOYEHAIYAOMOSUONONGDU。DANZAIMIANYIZUHUAZHONGWOMENYIBANXIANBAERKANGNONGDUHEFUYUSHIJIANXIANDINGXIA,RANHOUQUMOSUOYIKANGNONGDUHEFUYUSHIJIAN。

大神棋牌9、KANGTIXISHIYE:QISHIXUDUOSHIYANSHIKANGTIXISHIYEJIUYONGYIBANPBSJIKE,DANZHUANYONGDEKANGTIXISHIYEZHONGCHUPBSCHENGFENWAI,HAIJIALEDIEDAN-HUANAFANGFUJI、BSAWENDINGJIDENGZUFEN,DUIKANGTIDEDUOCIHUISHOULIYONGJIAOHAO。ZHENGYINWEIZHEIZHONGYUANYIN,WOYIZHIYONGGUOCHANDEZHUANYONGKANGTIXISHIYE,YIDUANSHIJIANZAIgengHUANXINKANGTIXISHIYESHISHIYANJIEGUOCHUXIANLEYINXINGJIEGUO(TISHIKENENGYIKANGMEIYOUJIEHE),ZUIHOUCONGKANGTINONGDUHEFUYUSHIJIAN、FENGBISHIJIANDENGYUANYINPAICHUHOU,FAXIANSHIXINKANGTIXISHIYEDEPHZHIPIANSUAN,ERSHIKANGYUANKANGTIFANYINGBUJIA,ZHONGERCHUXIANJIAYINXINGJIEGUO。

大神棋牌10、QIEPIANQINGXI(JINXI、CHONGXIHEPIAOXI):WEILEFANGZHIYIKANG、ERKANGDENGSHIJICANLIUERYINQIFEITEYIXINGRANSE,SUOYISHIDANGDIJIAQIANGQINGXI(YANZHANGSHIJIANHEZENGDUOCISHU)YOUWEIZHONGYAO,WOYIBANZAIYIKANGFUYUQIANDEQINGXISHI3min*3CI,ERYIKANGFUYUHOUDEQINGXIJUNWEI5CI*5min。

大神棋牌ZHUYI:(1)DANDUCHONGXI,FANGZHIJIAOCHAFANYINGZAOCHENGWURAN。(2)WENROUCHONGXI,FANGZHIQIEPIANDETUOLUO。WOXIHUANYONGJINXIFANGSHI;(3)CHONGXIDESHIJIANYAOZUGOU,CAINENGCHEDIXIQUJIEHEDEWUZHI。(4)PBSDEPHHELIZIQIANGDUDESHIYONGHEYAOQIU。ZHEIFANGMIANWOYOUCANTONGJIAOXUN,DANGSHIWOMAIDEKANGTIXISHIYEPIANSUAN,JIEGUOBEIJINGYIPIANHUANG(WEIJIANTEYIXINGRANSE),JIANYIPHZAI7.4-7.6NONGDUSHI0.01M。(ZHONGXINGJIRUOJIANXINGTIAOJIAN(PH7-8)YOULIYUMIANYIFUHEWUDEXINGCHENG,ERSUANXINGTIAOJIANZEYOULIYUFENJIE;DILIZIQIANGDUYOULIYUMIANYIFUHEWUDEXINGCHENG,ERGAOLIZIQIANGDUZEYOULIYUFENJIE)

大神棋牌11、DABXIANSE:BEIJINGDESHENQIANHETEYIXINGRANSEDESHENQIANJUNKEYIYOUDABFUYUTIAOJIANJUEDING。DABXIANSESHIJIANBUSHIGUDINGDE,ZHUYAOYOUXIANWEIJINGXIAKONGZHIXIANSESHIJIAN,DAOCHUXIANTEYIXINGRANSEJIAOQIANGERBENDIZHESEJIAOQIANSHIJIKECHONGXI;DABXIANSESHIJIANHENDUAN(RUJIMIAOHUOJISHIMIAO)JIUCHUXIANHENSHENDEZONGHESE,ZHEIHENKENENGSHUOMINGNIDEKANGTINONGDUGUOGAOHUOKANGTIFUYUSHIJIANGUOZHANG,XUYAOXIADIAOKANGTINONGDUHUOSUODUANNIDEKANGTIFUYUSHIJIAN;CIWAI,RUOHENDUANSHIJIANJIUCHUXIANBEIJINGHENSHEN,HAIYOUKENENGNIQIANMIANDEFENGBIFEITEYIXINGDANBAIBUQUAN,XUYAOYANZHANGFENGBISHIJIAN;DABXIANSESHIJIANHENZHANG(RUCHAOGUOSHIJIFENZHONG)CAICHUXIANYANGXINGRANSE,YIFANGMIANKENENGSHUOMINGNIDEKANGTINONGDUGUODIHUOFUYUSHIJIANGUODUAN(ZUI-HAOYIKANG4DUGUOYE);LINGYIFANGMIANJIUSHIFENGBISHIJIANGUOZHANG。

12、FURAN:MUDESHIXINGCHENGXIBAOLUNKUO,CONGERgengHAODIDUIMUBIAODANBAIJINXINGDINGWEI,JINGCHANGYONGSUMUSUFURAN(BAOHERANLIAO)。ZHUYISUMUSUFURANSHIJIANYAOKANDANGSHIDESHIWEN、RONGYEDEXINJIU、MUBIAOKANGYUANDEDINGWEIDENGQINGKUANG,YIBANSHUMIAO-SHUFENZHONG,BAOJIANGDANBAIKEYISHIDANGSHIJIANZHANGYIDIAN,ERBAOHEDANBAIZEYAODUAN。BUGUOZHEIGERUGUORANSEBULIXIANGKEYIBUJIUDE。JI:RANSESHENZEFENHUASHIJIANSHAOZHANGXIEJIKE;RANSEQIANZEZAIZHIYUSUMUSUZHONGRANSEJIKE。

YANSUANJIUJINGSHIFENHUA,ANSHUISHIFANLAN。ZUOYONGBUTONG。PIANZIFURANWANHOULIUSHUIZHENXI,RANHOUZHIYUYANSUANJIUJINGZHONGSHUMIAO(YIDINGDONGZUOYAOKUAI)HOUNACHULIUSHUIZHENXI,ZAIFANGRUANSHUIZHONGFANLANJIKE。

大神棋牌13、FENGPIAN:WEILEZHANGQIBAOCUN,WOMENYIBANYONGZHONGXINGSHUJIAODENGFENGPIAN,BIMIANCHANSHENGQIPAO,FANGFASHIZHIJIEZAIZAIBOPIANZUZHISHANGDIYIDIFENGPIANYE,RANHOUYISHOUNAZHUGAIPIANMOUYIGUAIJIAO,ERLINGYISHOUNADUIMIANDENEIGEGUAIJIAO,JIEJINFENGPIANYEJINDUANDEGUAIJIAOXIANJIANGDI,ZHIZHIJIECHUDAOYETISHIWEIZHI;DANGFAXIANYETIJIECHUMIANZAIBUDUANMISANSHI,ZEKEYIHUANMANJIANGDILINGYIGUAIJIAO,ZHEIYANGYIBANBUHUICHANSHENGQIPAO。

ER、MIANYIYINGGUANGFANGFAZHONGDEZHONGYAOHUANJIE

大神棋牌1、BINGDONGQIEPIANZHIBEI:JIANYIYONGXINXIANZUZHI,FOUZEZUZHIXIBAONEIBUJIEGOUPOHUAI,YISHIKANGYUANMISAN。XUANYONGGANJINGFENGLIDEDAOPIAN、ZUZHIYIDINGYAOLENGDONGSHIDUDENG,FANGZHILIEPIANHETUOPIANYANZHONG。

2、ZUZHIQIEPIANGUDING:QIEHAOPIANFENGGANHOULIJIYONGBINGBINGTONGDENGGUDINGYEJINXINGGUDING5-10min,YOUQIYAOJIAOZHANGSHIJIANBAOCUNDEBAIPIAN,YIDINGYAOJISHIGUDINGHESHIDANGBAOCUN。

3、XUEQINGFENGBI:WEIFANGZHINEIYUANXINGFEITEYIXINGDANBAIKANGYUANDEJIEHE,XUYAOZAIYIKANGFUYUQIANXIANYONGXUEQING(YUERKANGLAIYUANYIZHI)FENGBI,JIANRUOBEIJINGZHESE。XUEQINGFENGBIDESHIJIANSHIKEYIDIAOZHENGDE,YIBAN10-30min。

大神棋牌4、YIKANGFUYUTIAOJIAN:ZAIMIANYIZUHUAFANYINGZHONGZUIZHONGYAO,BAOKUOFUYUSHIJIANHEKANGTINONGDU。YIKANGFUYUWENDUYOUJIZHONG:4DU、SHIWEN、37DU,QIZHONG4DUXIAOGUOZUI-JIA;FUYUSHIJIAN:ZHEIYUWENDU、KANGTINONGDUYOUGUAN,YIBAN37DU1-2h,ER4DUGUOYEHECONGBINGXIANGNACHUHOU37DUFUWEN45min。JUTITIAOJIANHAIYAOMOSUO。

5、ERKANGFUYUTIAOJIAN:ERKANGYIBANSHIWENHUO37DU30min-1h,JUTISHIJIANXUYAOMOSUO,ERNONGDUYIBANYOUGONGZUOYE,RUOSHINONGSUOYEHAIYAOMOSUONONGDU,QIEJIYAOBIGUANGFANYING。DANZAIMIANYIYINGGUANGZHONGWOMENYIBANXIANBAERKANGNONGDUHEFUYUSHIJIANXIANDINGXIA,RANHOUQUMOSUOYIKANGNONGDUHEFUYUSHIJIAN。ZUIHOU,YINGGUANGSUBIAOJIDEERKANGSUIZHEBAOCUNSHIJIANDEYANZHANG,KENENGHOUYOUDALIANGDEYOULIYINGGUANGSUCANLIU,XUYAOZHUYIPEIZHISHIXIAOBAOZHUANGHEBINGJINXINGSHIDANGDELIXIN。

6、FURAN:MUDESHIXINGCHENGXIBAOLUNKUO,CONGERgengHAODIDUIMUBIAODANBAIJINXINGDINGWEI。YIBANCHANGYONGDAPIFURAN。

大神棋牌7、FENGPIAN:WEILEZHANGQIBAOCUN,WOMENYIBANYONGHUANCHONGGANYOUDENGFENGPIAN,CIWAIHAIYOUZHUANMENDEKANGYINGGUANGCUIMIEFENGPIANYE。BIMIANCHANSHENGQIPAO,FANGFASHIZHIJIEZAIZAIBOPIANZUZHISHANGDIYIDIFENGPIANYE,RANHOUYISHOUNAZHUGAIPIANMOUYIGUAIJIAO,ERLINGYISHOUNADUIMIANDENEIGEGUAIJIAO,JIEJINFENGPIANYEJINDUANDEGUAIJIAOXIANJIANGDI,ZHIZHIJIECHUDAOYETISHIWEIZHI;DANGFAXIANYETIJIECHUMIANZAIBUDUANMISANSHI,ZEKEYIHUANMANJIANGDILINGYIGUAIJIAO,ZHEIYANGYIBANBUHUICHANSHENGQIPAO。

8、QIEPIANQINGXI:WEILEFANGZHIYIKANG、ERKANGDENGSHIJICANLIUERYINQIFEITEYIXINGRANSE,SUOYISHIDANGDIJIAQIANGQINGXI(YANZHANGSHIJIANHEZENGDUOCISHU)YOUWEIZHONGYAO,WOYIBANZAIYIKANGFUYUQIANDEQINGXISHI3min*3CI,ERYIKANGFUYUHOUDEQINGXIJUNWEI5CI*5min。ZHUYI

(1)DANDUCHONGXI,FANGZHIJIAOCHAFANYINGZAOCHENGWURAN。

(2)WENROUCHONGXI,FANGZHIQIEPIANDETUOLUO。WOXIHUANYONGJINXIFANGSHI;

(3)CHONGXIDESHIJIANYAOZUGOU,CAINENGCHEDIXIQUJIEHEDEWUZHI。

(4)PBSDEPHHELIZIQIANGDUDESHIYONGHEYAOQIU。ZHEIFANGMIANWOYOUCANTONGJIAOXUN,DANGSHIWOMAIDEKANGTIXISHIYEPIANSUAN,JIEGUOBEIJINGYIPIANHUANG(WEIJIANTEYIXINGRANSE),JIANYIPHZAI7.4-7.6NONGDUSHI0.01M。(ZHONGXINGJIRUOJIANXINGTIAOJIAN(PH7-8)YOULIYUMIANYIFUHEWUDEXINGCHENG,ERSUANXINGTIAOJIANZEYOULIYUFENJIE;DILIZIQIANGDUYOULIYUMIANYIFUHEWUDEXINGCHENG,ERGAOLIZIQIANGDUZEYOULIYUFENJIE)

9、PAIZHAO:YOUTIAOJIANDEHUAZUI-HAOLIJIPAIZHAO,RUOBUNENGJISHIPAIZHAO,YEYAOFENGHAOPIANHEYONGZHIJIAYOUFENGGU,BAOCHIBIGUANGHESHIDU。SHIYONGYINGGUANGXIANWEIJINGZHUYIYANGEANZHAOYINGGUANGXIANWEIJINGCHUCHANGSHUOMINGSHUYAOQIUJINXINGCAOZUO,BUYAOSUIYIGAIBIANCHENGXU;YINGZAIANSHIZHONGJINXINGJIANCHA;FANGZHIZIWAIXIANDUIYANJINGDESUNHAI,ZAIDIAOZHENGGUANGYUANSHIYINGDAISHANGFANGHUYANJING;JIANCHASHIJIANMEICIYI1~2hWEIYI,CHAOGUO90min,CHAOGAOYAGONGDENGFAGUANGQIANGDUZHUJIANXIAJIANG,YINGGUANGJIANRUO;BIAOBENSHOUZIWAIXIANZHAOSHE3~5minHOU,YINGGUANGYEMINGXIANJIANRUOHUOTUNSE;JIFAGUANGZHANGSHIJIANDEZHAOSHE,HUIFASHENGYINGGUANGDESHUAIJIANHECUIMIEXIANXIANG;SUOYIZUIDUOBUDECHAOGUO2~3h;YINGGUANGXIANWEIJINGGUANGYUANSHOUMINGYOUXIAN,BIAOBENYINGJIZHONGJIANCHA,YIJIESHENGSHIJIAN,BAOHUGUANGYUAN。TIANRESHI,YINGJIADIANSHANSANREJIANGWEN,XINHUANDENGPAOYINGCONGKAISHIJIUJILUSHIYONGSHIJIAN。

大神棋牌DENGXIMIEHOUYUZAIQIYONGSHI,XUDAIDENGGUANGCHONGFENLENGQUEHOUCAINENGDIANRAN。YITIANZHONGYINGBIMIANSHUCIDIANRANGUANGYUAN。GUANBIGONGDENGZHISHAOZAIKAIQI15-30FENZHONGHOU;BIAOBENRANSEHOULIJIGUANCHA,YINSHIJIANJIULEYINGGUANGHUIZHUJIANJIANRUO。RUOJIANGBIAOBENFANGZAIJUYIXISULIAODAIZHONG4℃BAOCUN,KEYANHUANYINGGUANGJIANRUOSHIJIAN,FANGZHIFENGBIAOJIZHENGFA;SHIYONGDEBOPIANDENGZAITI,DOUBIXUHOUDUJUNYUN,WUMINGXIANDEZIFAYINGGUANG,RUGUOSHIYONGYOUJING,HAIBIXUBAOZHENGJINGYOUWEIWUYINGGUANGJINGYOU;DIANYUANZUI-HAOZHUANGWENYAQI,FOUZEDIANYABUWENBUJINHUIJIANGDIGONGDENGDESHOUMING,YEHUIYINGXIANGJINGJIANDEXIAOGUO。

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