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上海远慕生物科技有限公司

大肠杆菌质粒DNA的提取实验
点击次数:23 发布时间:2020-07-27

SHIYANFANGFAYUANLI:

大神棋牌JIANFATIQUZHUYAOSHILIYONGGONGJIABIHEHUANZHUANGZHILIYUXIANXINGRANSEZHIZAITUOPUXUESHANGDECHAYILAIFENLITAMEN。ZAIJIANXINGpHHUANJING,DNABIANXING,DANGHUIFUZHONGXINGBINGZAIGAOYANLIZINONGDUSHI,JUEDADUOSHUZHILIDNAKEYIZHUNQUEFUXING,LIUZAISHANGQINGZHONG;ERXIANXINGRANSETIDNABUNENGZHUNQUEFUXING,XIANGHUJIAOLIANCHANRAOFUZHEZAIXIBAOBISUIPIANSHANGYUDANBAIZHIFASHENGCHENDIAN。LIXINHOUHUODEDALIANGZHILIDESHANGQINGYE,LIYONGQINSHUIXINGYOUJIRONGJI(YICHUN、YIBING-CHUN、PEG8000)SHIZHILIDNATUOSHUI,LIXINHOUSHOUJI。

SHIYANCAILIAO :HANZHILIDEDACHANGGANJUN

大神棋牌SHIJI、SHIJIHE:PUTAOTANG EDTA Tris-HCl NaOH SDS KAc YIWU-CHUN

YIQI、HAOCAI:TAISHIGAOSULIXINJI HENGWENZHENDANGYAOCHUANG GAOYAMIEJUNGUO ZHENDANGQI WEILIANGYIYEQI 1.5mlLIXINGUAN

 

 

YI、SHIJIPEIZHI

1. LBYETIPEIYANGJI。

2. 100 mg/mlANBIANXILINCHUCUNYE:CHENGQU25gDEANBIANXILINFENMO,JIAQULIZISHUIZHI250ml,WANQUANRONGJIEHOUGUOLV、FENZHUANG,20℃BAOCUN。

3. RONGYEⅠ:50 mmol/LPUTAOTANGRONGYE,25 mmoi/L Tris-YANSUAN(pH 8.0),10 mmol/L EDTA(pH 8 0)。1 mol/L Tris-YANSUAN(pH 8.0)12.5ml,0 5 mol/L EDTA(pH8.0)10ml,PUTAOTANG4.730g;JIAQULIZISHUIZHI500ml,GAOYAMIEJUN,ZHUCUNYU4℃。

4. RONGYEⅡ:0.2moI/L QINGYANG-HUANARONGYE,1% SDS(10 N QINGYANG-HUANA20 μl,10%SDS 100μl,JIAQULIZISHUIZHI1ml),SHIYONGQIANLINSHIPEIZHI。

大神棋牌5. RONGYEⅢ(pH 4.8):5 mol/L CUSUANJIA300ml,BINGCU-SUAN57.5ml,JIAQULIZISHUIZHI500ml,4℃BAOCUN。

大神棋牌6. BEN-FEN:LV-FANG(1:1,V/V)(FENHELV-FANGJUNYOUHENQIANGDEFUSHIXINGCAOZUOSHIYINGDAISHOUTAO!)。

7. WUSHUIYICHUN。

8. 70%YICHUN。

大神棋牌9. RNase A(20 mg/ml)RONGYE:100 mg RNAMEIGANFENJIA5mlCHAOCHUNSHUIRONGJIE,RANHOUZAIFEISHUIZHU

大神棋牌15min,FENZHUANG,20℃BAOCUN。

大神棋牌10. TEHUANCHONGYE:10 mmol/L Tris-YANSUAN(pH8.0),1 mmol/L EDTA(pH8.O)。

ER、SHIYANFANGFA

1. TIAOQULBGUTIPEIYANGJISHANGSHENGZHANGDEDANJUNLUO,JIEZHONGYU2.0ml LB(HANAmp 100 μg/ml)YETIPEIYANGJIZHONG。37℃、250 r/minZHENDANGPEIYANGGUOYE(YUE12——14h)。

大神棋牌2. QU1.5mlXIJUNPEIYANGYEDAORULIXINGUANZHONG,4℃、6000ZHUANLIXIN30s,QISHANGQING,JIANGLIXINGUANDAOZHIYUZHIJINSHANG,QUCHUCANLIUYETI。

大神棋牌3. JUNTICHENDIANZHONGXUANYU100μlYONGBINGYULENGDERONGYEⅠZHONG,JULIEZHENDANG,SHIJUNTIFENSANHUNYUN,SHIWENXIAFANGZHI5 min。

大神棋牌4. JIARU200μlXINPEIZHIDERONGYEⅡ,GAIJINGUANKOU,KUAISUWENHEDIANDAOLIXINGUAN5——10CI,YIHUNYUNNEIRONGWU,BINGYU5min。

5. JIARU150μlYULENGDERONGYEⅢ,GAIJINGUANKOU,WENHEDIANDAO5——10CIHUNYUN,BINGYU3——5min,4℃、6000ZHUANLIXIN5min。ZHUANYISHANGQINGDAOYIGEXINDE1.5ml EpGUANZHONG。

6. JIARUDENGTIJIDEFENYILV-FANGCHOUTIJI,ZHENDANGHUNYUN,4℃、6000ZHUAN,LIXIN2 rain。

大神棋牌7. XIAOXINYICHUSHANGQINGYU1.5ml EpGUANZHONG;JIARU2BEITIJIYONGBINGYULENGDEWUSHUIYICHUNYUSHIWENCHENDIANSHUANGLIANDNA,HUNYUN,SHIWENFANGZHI2--5rain,4℃、6000ZHUANLIXIN5min。

8. QISHANGQING,JIANGLIXINGUANDAOZHIYUZHIJINSHANGSHISUOYOUYETILIUCHU,JIARU1ml 70%YICHUN,GAIJINLIXINGUANDIANDAOSHUCI,4℃、6000ZHUAN,LIXIN2min。

大神棋牌9. QUCHUSUOYOUDEYICHUNRONGYE,JIANGGUANDAOZHIYUZHIJINSHANGSHIYETILIUJIN,SHIWENGANZAO5——10min,ZHIDAOYICHUNDOUHUIFAGANJING。

大神棋牌10.JIANGZHILIDNACHENDIANRONGYU50 μl TEHUANCHONGYE(HAN20 μg/ml RNaseA)ZHONG,WENHEZHENDANGSHUMIAO,-20℃CHUCUNBEIYONG。

ZHUYISHIXIANG:

大神棋牌1. RONGYEⅠYURONGJUNYECHONGFENYAOYUN,YONGLISHIDANG。YINWEICISHIXIJUNHAIMEIYOUYUJIANHESDSZUOYONG,RANSETIDNASHANGWEISHIFANG,BUBIDANXINQIFENZIDUANLIE,YIBANKEYONGLIZHENDANGJICI。

2. JIARUSDSHOUZEYAOZHUYIBUNENGGUOFENYONGLIZHENDANG,WENHEHUNYUN,DANYOUBIXURANGTAFANYINGCHONGFEN。

3. JIARURONGYEⅡHUNYUNZHIHOU,YIDINGYAOZAIBINGSHANGFANGZHI,BUYAOYAODONG,DUIYURONGYEⅢTONGYANGCHULI。

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