大神棋牌

上海远慕生物科技有限公司

远慕生物:一些生化试剂的原理
点击次数:44 发布时间:2019-11-28

1.RONGYEI—RONGJUNYE:

大神棋牌RONGJUNMEI:TASHITANGGANSHUIJIEMEI,NENGSHUIJIEJUNTIXIBAOBIDEZHUYAOHUAXUECHENGFENTAIJUTANGZHONGDEβ-1,4TANGGANJIAN,YINERJUYOURONGJUNDEZUOYONG。DANGRONGYEZHONGpHXIAOYU8SHI,RONGJUNMEIZUOYONGSHOUDAOYIZHI。

PUTAOTANG:ZENGJIARONGYEDEZHANDU,WEICHISHENTOUYA,FANGZHIDNASHOUJIXIEJIANQIELIZUOYONGERJIANGJIE。

EDTA:(1)AOHEMg2+、Ca2+DENGJINSHULIZI,YIZHITUOYANGHETANGHESUANMEIDUIDNADEJIANGJIEZUOYONG(DNaseZUOYONGSHIXUYAOYIDINGDEJINSHULIZIZUOFUJI);(2)EDTADECUNZAI,YOULIYURONGJUNMEIDEZUOYONG,YINWEIRONGJUNMEIDEFANYINGYAOQIUYOUJIAODIDELIZIQIANGDUDEHUANJING。

 

2.RONGYEII-NaOH-SDSYE:

大神棋牌NaOH:HESUANZAIpHDAYU5,XIAOYU9DERONGYEZHONG,SHIWENDINGDE。DANDANGpH>12HUOpH<3SHI,JIUHUIYINQISHUANGLIANZHIJIANQINGJIANDEJIELIERBIANXING。ZAIRONGYEIIZHONGDENaOHNONGDUWEI0.2mo1/L,JIACHOUTIYESHI,GAIXITONGDEpHJIUGAODA12.6,YINERCUSHIRANSETIDNAYUZHILIDNADEBIANXING。

SDS:SDSSHILIZIXINGBIAOMIANHUOXINGJI。TAZHUYAOGONGNENGYOU:(1)RONGJIEXIBAOMOSHANGDEZHIZHIYUDANBAI,YINERRONGJIEMODANBAIERPOHUAIXIBAOMO。(2)JIEJUXIBAOZHONGDEHEDANBAI。(3)SDSNENGYUDANBAIZHIJIEHECHENGWEIR-O-SO3-…R+-DANBAIZHIDEFUHEWU,SHIDANBAIZHIBIANXINGERCHENDIANXIALAI。DANSHISDSNENGYIZHIHETANGHESUANMEIDEZUOYONG,SUOYIZAIYIHOUDETIQUGUOCHENGZHONG,BIXUBATAQUCHUGANJING,FANGZHIZAIXIAYIBUCAOZUOZHONG(YONGRNaseQUCHURNASHI)SHOUDAOGANRAO。

 

3. RONGYEIII--3mol/L NaAc(pH4.8)RONGYE:

大神棋牌NaAcDESHUIRONGYECHENGJIANXING,WEILEDIAOJIEpHZHI4.8,BIXUJIARUDALIANGDEBINGCUSUAN。SUOYIGAIRONGYESHIJISHANGSHINaAc-HAcDEHUANCHONGYE。YONGpH4.8DENaAcRONGYESHIWEILEBApH12.6DECHOUTIYE,DIAOHUIpHZHIZHONGXING,SHIBIANXINGDEZHILIDNANENGGOUFUXING,BINGNENGWENDINGCUNZAI。ERGAOYANDE3mol/L NaAcYOULIYUBIANXINGDEDAFENZIRANSETIDNA、RNAYIJISDS-DANBAIFUHEWUNINGJUERCHENDIANZHI。QIANZHESHIYINWEIZHONGHEHESUANSHANGDEDIANHE,JIANSHAOXIANGCHILIERHUXIANGJUHE,HOUZHESHIYINWEINAYANYUSDS-DANBAIFUHEWUZUOYONGHOU,NENGXINGCHENGJIAOXIAODENAYANXINGSHIFUHEWU,SHICHENDIANGENGWANQUAN。

 

4.WEISHENMEYONGWUSHUIYICHUNCHENDIANDNA?

大神棋牌YONGWUSHUIYICHUNCHENDIANDNA,ZHEISHISHIYANZHONGCHANGYONGDECHENDIANDNADEFANGFA。YICHUNDEYOUDIANSHIKEYIRENYIBIHESHUIXIANGHUNRONG,YICHUNYUHESUANBUHUIQIRENHEHUAXUEFANYING,DUIDNAHENANQUAN,YINCISHILIXIANGDECHENDIANJI。

大神棋牌DNARONGYESHIDNAYISHUIHEZHUANGTAIWENDINGCUNZAI,DANGJIARUYICHUNSHI,YICHUNHUIDUOQUDNAZHOUWEIDESHUIFENZI,SHIDNASHISHUIERYIYUJUHE。YIBANSHIYANZHONG,SHIJIA2BEITIJIDEWUSHUIYICHUNYUDNAXIANGHUNHE,QIYICHUNDEZHONGHANLIANGZHAN67%ZUOYOU。YINERYEKEGAIYONG95%YICHUNLAITIDAIWUSHUIYICHUN(YINWEIWUSHUIYICHUNDEJIAGEYUANYUANBI95%YICHUNANGGUI)。DANSHIJIA95%DEYICHUNSHIZONGTIJIZENGDA,ERDNAZAIRONGYEZHONGYOUYIDINGCHENGDUDERONGJIE,YINERDNASUNSHIYEZENGDA,YOUQIYONGDUOCIYICHUNCHENDIANSHI,JIUHUIYINGXIANGSHOUDELV。ZHEZHONGDEZUOFASHICHUCICHENDIANDNASHIKEYONG95%YICHUNDAITIWUSHUIYIJIAO,HOUDECHENDIANBUZHOUYAOSHIYONGWUSHUIYICHUN。YEKEYIYONG0.6BEITIJIDEYIBINGCHUNXUANZEXINGCHENDIANDNA。YIBANZAISHIWENXIAFANGZHI15-30FENZHONGJIKE。

 

大神棋牌5.ZAIYONGYICHUNCHENDIANDNASHI,WEISHENMEYIDINGYAOJIANaAcHUONaClZHIZHONGNONGDUDA0.1~0.25mol/L?

ZAIpHWEI8ZUOYOUDERONGYEZHONG,DNAFENZISHIDAIFUDIANHEDE,JIAYIDINGNONGDUDENaAcHUONaCl,SHINa+ZHONGHEDNAFENZISHANGDEFUDIANHE,JIANSHAODNAFENZIZHIJIANDEtongXINGDIANHEXIANGCHILI,YIYUHUXIANGJUHEERXINGCHENGDNANAYANCHENDIAN,DANGJIARUDEYANRONGYENONGDUTAIDISHI,ZHIYOUBUFENDNAXINGCHENGDNANAYANERJUHE,ZHEIYANGJIUZAOCHENGDNACHENDIANBUWANQUAN,DANGJIARUDEYANRONGYENONGDUTAIGAOSHI,QIXIAOGUOYEBUHAO。ZAICHENDIANDEDNAZHONG,YOUYUGUODUODEYANZAZHICUNZAI,YINGXIANGDNADEMEIQIEDENGFANYING,BIXUYAOJINXINGXIDIHUOZHONGCHENDIAN。

 

大神棋牌6.JIAHETANGHESUANMEIJIANGJIEHETANGHESUANHOU,WEISHENMEZAIYAOYONGSDSYUKAcLAICHULI?

大神棋牌JIAJINQUDERNaseBENSHENSHIYIZHONGDANBAIZHI,WEILECHUNHUADNA,YOUBIXUQUCHUZHI,JIASDSKESHITAMENCHENGWEISDS-DANBAIFUHEWUCHENDIAN,ZAIJIAKAcSHIZHEIXIEFUHEWUZHUANBIANWEIRONGJIEDUGENGXIAODEJIAYANXINGSHIDESDS-DANBAIZHIFUHEWU,SHICHENDIANGENGJIAWANQUAN。YEKEYONGBAOHEFEN、LVfangCHOUTIZAICHENDIAN,QUCHURNase。ZAIRONGYEZHONG,YOURENYIKAcDAITINaAc,YEKEYISHOUDAOJIAOHAOXIAOGUO。

 

大神棋牌7.WEISHENMEZAIBAOCUNHUOCHOUTIDNAGUOCHENGZHONG,YIBANCAIYONGTEHUANCHONGYE?

ZAIJIYINCAOZUOSHIYANZHONG,XUANZEHUANCHONGYEDEZHUYAOYUANZESHIKAOLVDNADEWENDINGXINGJIHUANCHONGYECHENGFENBUCHANSHENGGANRAOZUOYONG。LINSUANYANHUANCHONGXITONG(pKa=7.2)HEPENGSUANXITONG(pKa=9.24)DENGSUIRANYEDOUFUHEXIBAONEIHUANJINGDESHENGLIFANWEI(pH),KEZUODNADEBAOCUNYE,DANZAIZHUANHUASHIYANSHI,LINSUANGENLIZIDEZHONGLEIJISHULIANGJIANGYUCa2+CHANSHENGCa3(PO4)2CHENDIAN;ZAIDNAFANYINGSHI,BUTONGDEMEIDUIFUZHUYINZIDEZHONGLEIJISHULIANGYAOQIUBUTONG,YOUDEYAOQIUGAOLIZINONGDU,YOUDEZEYAOQIUDIYANNONGDU,CAIYONGTris-HCl(pKa=8.0)DEHUANCHONGXITONG,YOUYUHUANCHONGYESHITrisH+/Tris,BUCUNZAIJINSHULIZIDEGANRAOZUOYONG,GUZAITIQUHUOBAOCUNDNASHI,DADOUCAIYONGTris-HClXITONG,ERTEHUANCHONGYEZHONGDEEDTAGENGNENGWENDINGDNADEHUOXING。

 

8.RUHEXUANZEJUYIERCHUN(6000)DENONGDULAICHENDIANDNA?

CAIYONGPEG(6000)CHENDIANDNA,DAXIAOBUTONGDEDNAFENZISUOYONGDEPEGDENONGDUYEBUTONG,PEGDENONGDUDI,XUANZEXINGCHENDIANDNAFENZILIANGDA,DAFENZISUOXUPEGDENONGDUZHIXU1%ZUOYOU,XIAOFENZISUOXUPEGNONGDUGAODA20%。BENSHIYANXUANZEXINGCHENDIAN4.3kbDEpBR322ZHILIDNA,MEIHAOSHENGJIARU0.4HAOSHENGDE30% PEG,QIZHONGPEGNONGDUWEI12%。PEGXUANZEXINGCHENDIANDNADEFENBIANLVDAYUE100bp。

 

9.CHOUTIDNAQUCHUDANBAIZHISHI,ZENYANGSHIYONGFENYULVfangJIAOHAO?

TAIYULVfangSHIFEIJIXINGFENZI,SHUISHIJIXINGFENZI,DANGDANBAISHUIRONGYEYUFENHUOLVfangHUNHESHI,DANBAIZHIFENZIZHIJIANDESHUIFENZIJIUBEIFENHUOLVfangJIQU,SHIDANBAISHIQUSHUIHEZHUANGTAIERBIANXING。JINGGUOLIXIN,BIANXINGDANBAIZHIDEMIDUBISHUIDEMIDUWEIDA,YINERYUSHUIXIANGFENLI,CHENDIANZAISHUIXIANGXIAMIAN,CONGERYURONGJIEZAISHUIXIANGZHONGDEDNAFENKAI。ERFENYULVfangYOUJIRONGJIBIZHONGGENGDA,BAOLIUZAIXIACENG。

ZUOWEIBIAOMIANBIANXINGDEFENYULVfang,ZAIQUCHUDANBAIZHIDEZUOYONGZHONG,GEYOULIBI,FENDEBIANXINGZUOYONGDA,DANFENYUSHUIXIANGYOUYIDINGCHENGDUDEHURONG,DAYUE10%~15%DESHUIRONGJIEZAIFENXIANGZHONG,YINERSUNSHILEZHEIBUFENSHUIXIANGZHONGDEDNA,ERLVfangDEBIANXINGZUOYONGBURUFENXIAOGUOHAO,DANLVfangYUSHUIBUXIANGHUNRONG,BUHUIDAIZOUDNA。SUOYIZAICHOUTIGUOCHENGZHONG,HUNHESHIYONGFENYULVfangXIAOGUOhao。JINGFENCICHOUTIHOUDESHUIXIANGZHONGYOUCANLIUDEFEN,YOUYUFENYULVfangSHIHURONGDE,KEYONGLVfangDIERCIBIANXINGDANBAIZHI,CISHIYIQIJIANGFENDAIZOU。YEKEYIZAIDIERCICHOUTISHI,JIANGFENYULVfangHUNHE(1:1)SHIYONG。

 

大神棋牌10.WEISHENMEYONGFENYULVfangCHOUTIDNASHI,HAIYAOJIASHAOLIANGDEYIWUJIAO?

ZAICHOUTIDNASHI,WEILEHUNHEJUNYUN,BIXUJULIEZHENDANGRONGQISHUCI,ZHEISHIZAIHUNHEYENEIYICHANSHENGQIPAO,QIPAOHUIZUZHIXIANGHUJIANDECHONGFENZUOYONG。JIARUYIWUCHUNNENGJIANGDIFENZIBIAOMIANZHANGLI,SUOYINENGJIANSHAOCHOUTIGUOCHENGZHONGDEPAOMOCHANSHENG。YIBANCAIYONGLVfangYUYIWUJIAOWEI24:1ZHIBI。YEKECAIYONGFEN、LVfangYUYIWUCHUNZHIBIWEI25:24:1(BUBIXIANPEIZHI,KEZAILINYONGQIANBAYIFENFENJIAYIFEN24:1DELVfangYUYIWUCHUNJICHENG),TONGSHIYIWUCHUNYOUZHUYUFENXIANG,SHILIXINHOUDESHANGCENGSHUIXIANG,ZHONGCENGBIANXINGDANBAIXIANGYIJIXIACENGYOUJIRONGJIXIANGWEICHIWENDING。

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主营:ELISA试剂盒/染色液/溶液 /动物血清/标准品/化学试剂
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